Figure 1.

Steps taken during the experiment. (a,b) Isolation and expansion of MSCs from various tissues; (c) Infection of MSCs with retrovirus carrying HSVTK suicide gene; (d) Selection of cell population of suicide gene-transduced cells; (e) Harvesting of conditional medium; (f) Isolation of exosomes from conditional medium (CM) by size-exclusion chromatography; (g) Detection of mRNA of HSVTK suicide gene in the cargo of exosomes; (h) Tumor cell growth inhibition with CM and HSVTK-exosomes. Abbreviations: MSCs, mesenchymal stem cells; DP-MSCs, dental pulp MSCs; AT-MSCs, adipose-tissue MSCs; BM-MSCs, bone marrow MSCs; UC-MSCs, umbilical cord MSCs; HSVTK, thymidine kinase of herpes simplex virus gene.