Figure 4.

Tubulin-polymerization assay. The effect of compounds AuL4 and AuL7 on in vitro tubulin polymerization was evaluated. Two positive control molecules, Paclitaxel and Vinblastine, were used as tubulin-stabilizing and tubulin-destabilizing agents, respectively. DMSO was used as a negative control. Assembly of tubulin into microtubules was determined by measuring the turbidity at 350 nm for 3600 s at 37 °C. All the compounds were used at a concentration of 10 μM.