ROS induction by AuL7 treatment in MDA-MB-231 cells. (a) ROS production was evaluated by using DCF-DA in MDA-MB-231 cells treated with Menadione (Men, used as positive control), AuL7, or vehicle. Images were acquired at 20× magnification. (A): Nuclear stain with DAPI (excitation/emission wavelength 350 nm/460 nm) in treated (Men or AuL7) and untreated cells (vehicle, CTRL); (B): DCF (excitation/emission wavelength 490 nm/515 nm for CFQ488A) in treated (Men or AuL7) and untreated cells (vehicle, CTRL); (C): overlay channel; (b) Fluorescence quantification. *p < 0.001, AuL7 vs. Men. Fields are representative of three separate experiments.