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. 2020 Apr 30;12(5):1126. doi: 10.3390/cancers12051126

Figure 3.

Figure 3

LDI enhances the abscopal effect combined with high-dose RT. (A) Schematic representation of the X-RAD SmART (small animal image-guided radiation therapy research system; Precision X-ray Inc., North Branford, CT, USA) irradiator setup. Acquiring cone beam computed tomography (CBCT) scans to precisely place the isocenter to the treatment location and visualize the internal structure of mice. X-RAD SmART Plan to calculate dose, provides complex beam planning. (B) Experimental scheme of tumor inoculation and irradiation interval to mice as described in the Methods section. (C) Tumor growth was monitored with irradiated and abscopal tumors in each group. (D) Body and spleen weight were measured at the end of the experiment. Splenocyte number was determined using a Trypan blue exclusion assay. Whole blood was drawn by retro-orbital puncture and analyzed using a VETSCAN HM5 hematology analyzer (Abaxis, Union City, CA, USA). Data represent means ± SEM of 6-8 mice per experiment. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control, † p < 0.05, †† p < 0.01, ††† p < 0.001 vs. LDI, # p < 0.05, ### p < 0.001 vs. XRAD.