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. 2020 May 21;12(5):1299. doi: 10.3390/cancers12051299

Figure 4.

Figure 4

Influence of HuR knockdown on the proliferative capacity of melanoma cells in vitro. (A) Exemplary real-time cell proliferation curves of Mel Wei and Mel Im cells (left panel) and quantified ‘slope’ (proliferative ability, siCtrl = 1) (right panel). (B) Exemplary images of anchorage-dependent clonogenic assays of Mel Wei and Mel Im cells (left panel) and quantification of colony number and size (right panel) in these cells (siCtrl = 1). (C) FACS-based cell cycle analysis of Mel Wei and Mel Im cells. Depicted are representative overlays of cell cycle histograms (left panel) and percentages of G1 cell cycle fractions (right panel) in these cells (siCtrl = 1). (D) Luciferase AP-1-reporter activity in siCtrl and siHuR transfected melanoma cells (siCtrl = 1). (E) Densitometric quantification (right) and exemplary image (left) of Western blot analysis of Cyclin D1 protein levels in transfected Mel Wei and Mel Im cells (siCtrl = 1). *A p-value of <0.05 was considered statistically significant. The uncropped blots and molecular weight markers of Figure 4E are shown in Figure S8.