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. 2020 May 21;12(5):1299. doi: 10.3390/cancers12051299

Figure 6.

Figure 6

HuR overexpression leads to a reduction of senescence characteristics in NHEM BrafV600E cells. (A) Exemplary images of light microscopic examination of SA-β-galactosidase staining in NHEM Mock, NHEM BrafV600E, and NHEM BrafV600E/HuR OE cells (left). The percentages of SA-β-galactosidase positive cells (blue) were calculated (right) (Mock = 1). (B) Exemplary images of PML immunofluorescence staining of NHEM Mock, NHEM BrafV600E, and NHEM BrafV600E/HuR OE cells (red) and DAPI (blue) staining. CopGFP served as a transduction control (green) (left). The graph shows the nuclear accumulation of PML (Mock = 1) (right). For better recognition, colors are only shown in the overlay, not in single pictures. (C Exemplary images of Ki-67 immunofluorescence staining of NHEM Mock, NHEM BrafV600E, and NHEM BrafV600E/HuR OE cells (red) and DAPI (blue) staining. CopGFP served as a transduction control (green) (top). The graph shows the accumulation of Ki-67 (Mock = 1) (bottom left). For better recognition, colors are only shown in the overlay, not in single pictures. *A p-value of <0.05 was considered statistically significant.