Figure 4.

GSTA2, ME1 and FOXP2 show higher expression levels in the fimbria compared to the ampulla. (A) Immunofluorescence (IF) of the fimbria. The top panel shows a view of fimbria tissue at 10× magnification stained for GSTA2, ME1, FOXP2, PAX8 and ac-TUBULIN. In each panel an inset shows cells derived from fresh fimbria tissue, which were cultured on chamber slides, stained with the above markers and visualized at 63× magnification. Pax8 was used to identify secretory cells and acetylated-tubulin was used to show ciliated cells. Insets, i: FoxP2 and Pax8 localization in an FTE cell, ii: ME1 and acetylated-tubulin are found in the cytoplasm of cells and iii: GSTA2 and acetylated-tubulin expression in cells from normal FTE. GSTA2 is localized to the nucleus of FTE cells. (B) Immunoblot of two independent fresh fimbria and ampulla tissues from normal cases shows that GSTA2, FoxP2 and ME1 are highly expressed in fimbria compared to the ampulla. (C) IF was used to quantify the number of cells expressing GSTA2, FoxP2 and ME1 in the follicular and luteal phases. Scale bar: 100 µm. The uncropped blots and molecular weight markers of Figure 4B are shown in Figure S3.