Figure 4.
Effect of VE-821 and olaparib combination on replication stress. (A) pCHK1S345, pCHK2T68, γH2AX protein expression of NGP, N20_R1, SHSY5Y and SKNAS cells after incubation with 5 μM olaparib and/or 1 µM VE-821 for 24 h. (B) pCHK1S345, pCHK2T68, γH2AX mean fold change in protein band intensity measured by densitometry (ImageJ) and normalised to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) loading control, data are mean + SEM from 3 independent experiments. (C) pRPA2S8 expression in SHSY5Y cell line in response to 5 μM olaparib with or without 1 µM VE-821 over 48 h. Images of the uncropped Western blots can be found in Figure S8.