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Primers used in this study.
| Primers | Sequence (5’-3’) | DNA Template | Restriction Site |
|---|---|---|---|
| FP-nptII | AGATGGATTGCACGCAGG | pBSL180 | - |
| RP-nptII | CCACAGTCGATGAATCCAGA | Tn10 transposon | - |
| nptII-iF | GCCTTCTATCGCCTTCTTGAG | pBSL180 | - |
| nptII-iR | TACGTGTTCCGCTTCCTTTAG | pBSL180 | - |
| 0493F | GGATCCCACCCAAGGAAGTATTAAG | ATCC 17978 | BamHI |
| 0493R | GTCGACTGACACTTTGATGATAAATGC | ATCC 17978 | SalI |
| FP-0493 | GGATCCGCTGTTTCTCAATCGATCC | ATCC 17978 | BamHI |
| RP-0493 | GTCGACGTTCTGCAATTTCGCATA | ATCC 17978 | SalI |
Underline: Primers.
