Figure 2.

(A) Immunoblotting of the whole cell lysate (WCL) after trypsinization, conditioned media (CM), and extracellular matrix (ECM) of the 15 breast cancer (BC) cell lines indicated after 72 h in culture. None of these lines could produce fibrillar FN as an ECM. However, intracellular FN and soluble FN released into the media were detected from the majority of BC lines. Human lung fibroblasts (HLF) and mammary epithelial cells overexpressing transglutaminase (HMLE-TGM2) were used as positive controls for matrix deposition. (B) Immunofluorescent staining for FN in decellularized monolayers, performed in duplicate, showed limited FN matrix production by the BC cell lines as compared to HLF and HMLE-TGM2 cells. Scale bar is 50 µm.