Figure 7.

(A) FN levels in extracellular vesicle(EV)-enriched solutions from Ca1h, Ca1h shFN30, and Ca1a BC cells. (B) Representative images of FN matrices by HLFs after conditioning with EV-enriched solutions (n = 4). BC EVs affected the amount and orientation of fibrillar FN deposited by HLFs after 48 h. Scale bar is 50 µm. (C) Ca1h EV conditioning resulted in aligned FN fibrils by HLFs. Alignment was quantified from at least 3 images of high FN accumulation for each condition. (D) Ca1h and Ca1h shFN30 EVs resulted in significantly higher FN accumulation, while Ca1a EV conditioning produced no statistically significant effect. Accumulation (bar, solid) was quantified from whole-plate tilescans of duplicate experiments. Conditioning HLFs with EVs from Ca1h and Ca1h shFN30 cells resulted in significantly more HLFs after 48 h (scatter, dashed). (* indicates p < 0.05).