Growth inhibitory effect of C. sinensis on A. castellanii trophozoites at 24, 48, 72 and 96 h after treatment with 25%, 50%, 75% and 100% of C. sinensis. Significant differences were detected at all time points when control was compared to 25%, 50%, 75% and 100% (p < 0.0001). The 25% and 50% concentrations showed an increased in trophozoite numbers between 24 and 96 h. There was a progressive decrease in trophozoite number for 75% and 100% concentrations between 24 and 96 h with no significant difference between the effects of 75% and 100% (p > 0.05). (****, p < 0.0001; ns, p > 0.05).