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. 2020 Jun 2;13:59–72. doi: 10.1016/j.ijpddr.2020.05.001

Fig. 5.

Fig. 5

Scanning electron micrographs of A. castellanii treated with C. sinensis. Continued exposure to both chlorhexidine (CHX) and hot C. sinensis showed loss of acanthopodia and progressive destruction of A. castellanii trophozoites. (A) A group of trophozoites cultured in PYG medium (control) attached together and adherent to the surface; (B) Closer view of two trophozoites in PYG (C) Trophozoites in CHX for 24 h. (D) Trophozoites in hot C. sinensis for 24 h are misshaped with cell membrane disruption; (E) Membrane perforations and loss of integrity in trophozoites treated with CHX for 72 h; (F) Trophozoites in hot C. sinensis brew for 72 h are misshaped and shrunken. With increased duration of exposure to C. sinensis trophozoites became less adherent to the surface and had reduced cell volume possibly in the attempt to encyst. Magnifications: X2,000, X4,000, X500, X2,000, X800 and X3,000 for A to F, respectively. Scale bars = 10, 5, 50,10, 20 and 5 μm for A to F, respectively.