Figure 4.

miR-543 Inhibits Breast Cancer Metastasis by Regulation of ZNF281

(A) The expression of miR-543 in miR-543- or ZNF281/miR-543-overexpressed MDA-MB-231, as well as the control cells determined by qRT-PCR. (B) Morphology of cells as in (A). (C) The protein expression levels of epithelial markers E-cadherin and ZO-1, mesenchymal markers N-cadherin and Vimentin, and ZNF281 in the cells described in (A) were evaluated by western blotting. (D) Transwell analysis of cell invasion in cells as in (A). (E) Immunofluorescence analyses of epithelial marker E-cadherin and mesenchymal marker Vimentin in the cells as in (A). (F) The mRNA expression levels of epithelial markers E-cadherin and ZO-1 and mesenchymal markers N-cadherin and Vimentin in the cells described in (A) were evaluated by qRT-PCR. ∗∗∗p < 0.001.