Figure 5.

ZNF281 Transactivates the Snail and ZEB1 Expression

(A) The expression of EMT-related factors in ZNF281-overexpressed MCF10A and control cells. (B) Schematic depiction of the ZEB1 and SNAIL promoter. Black bars indicated primers for ChIP analysis. (C and D) ChIP analysis of interaction between ZNF281 and the ZEB1 (C) or Snail (D) promoter in ZNF281-transfected MCF10A and control cells using anti-HA or anti-IgG antibodies. Results represent the percentage of input chromatin. (E and F) Dual-luciferase analysis of ZEB1 (E) or Snail (F) promoter activity in ZNF281-transfected 293FT and control cells. ∗∗∗p < 0.001, ∗p < 0.01.