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. 2020 May 21;21:98–107. doi: 10.1016/j.omtn.2020.05.020

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© 2020 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

ZNF281-miR-543 Feedback Loop Is Involved in TGF-β-Induced EMT

(A) The expression of miR-543 in MCF10A cells after treatment with TGF-β1 at indicated concentration. (B) Transwell analysis of miR-543-overexpressed MCF10A or control cells with TGF-β1 and/or ZNF281 overexpression. (C) Luciferase reporter analysis of TGF-β signaling activity in cells as in (B). (D) Localization of SMAD2 in cells as in (B) as determined by immunofluorescence staining. (E) The expression of pSMAD2 in cells as in (B) by western blotting. (F) A model for the ZNF281-miR-543 feedback loop in TGF-β-induced breast cancer metastasis.