Fig. 6.

Knockdown of FOXA1 in 14q-amplified cell lines reveals a correlation between FOXA1 function and cellular respiration. (A) Top GSEA result for differentially expressed genes after FOXA1 knockdown in both H1819 and H1781, showing enrichment of electron transport gene expression in shNTC control samples as compared to shFOXA1 samples. (B) Representative dose response curves to phenformin treatment, in three 14q-amplified NSCLC cell lines (top panel), and three NSCLC cell lines without amplification (bottom panel). Profileration was measured by MTS, normalized to the lowest dose. Mean values are calculated from 8 technical replicates. Error bars indicate standard deviation. Data were fit to non-linear regression models using three parameters. (C) Overlay of all drug response curves in a panel of 11 NSCLC cell lines (pink = 14q amplified, black = non-amplified). (D) Correlation between FOXA1 copy number and phenformin IC50s, with select cell lines highlighted.