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. 2020 Apr 29;12(5):1108. doi: 10.3390/cancers12051108

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Phosphoproteomics analysis identified up- and downregulated candidates in acquired trastuzumab-resistant BT-474.r2T cells compared to parental sensitive BT-474 cells. Proteins selected in the phosphoproteomic SILAC assay according to their significantly downregulated or overexpressed phosphosites in BT-474.r2T vs. BT-474 cells (overlapping of the three datasets). Bar graphs represent FC of Class-1 phosphosites identified in different temporary conditions, with annotation of the modified residue.