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. 2020 Apr 29;12(5):1108. doi: 10.3390/cancers12051108

Figure 4.

Figure 4

TEAD1 and TEAD2 are highly expressed in trastuzumab-resistant BT-474.r2T cells and, together with YAP1, are upregulated in response to acquired trastuzumab resistance. 2.5E6 cells were seeded in 6-multiwell plates, and after 96 h of treatment, whole cell protein isolation, nuclear-cytoplasm protein fractionation, or RNA isolation were performed. (A) qPCR analysis of relative YAP1, TAZ, TEAD1, TEAD2, TEAD3, TEAD4, AREG, CTGF, CYR61, and VEGFA mRNA expression following in vitro generation of trastuzumab-resistant BT-474.r2T cells (*: p < 0.05, **: p < 0.01, ***: p < 0.001). (B) WB analysis of total YAP1, pYAP1-Ser109, TAZ, TEAD1, and TEAD2 expression in cells shown in (A). (C) WB analysis of total YAP, pYAP-Ser109, TAZ, TEAD1 and TEAD2 expression in the nuclear/cytosol extracts of cells shown in (A). Relative abundance levels of protein up- or downregulation were determined by densitometric analysis of the images.