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. 2020 May 21;12(5):1305. doi: 10.3390/cancers12051305

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Fibronectin-mediated interaction between cancer cells and PSCs migrating out of the collagen matrix channel. (A) Distance and number of migrating PANC-1 or BxPC-3 cells under PSC co-culture conditions. Cells that migrated beyond 100 µm from the respective channels were included in the analysis. (B) Cancer cells adhering to fibronectin fibers of spindle-shape PSCs. Scale bar: 20 µm. White arrowhead or C: cancer cells. Yellow arrowhead or P: PSCs. Arrows indicate fibronectin fibers secreted by PSCs. Three fields covering 80% of the effective area in each channel were imaged per experiment and subjected to analysis. Data were obtained from three separate independent experiments. *** p < 0.001.