Figure 3.

Effects of compound 2 on the protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-induced BV2 (A) and RAW264.7 cells (B). Cells were pre-treated with compound 2 (1.0, 2.0, and 4.0 μM) for 3 h and stimulated with LPS (1 μg/mL) for 24 h. The representative blots of three independent analyses for iNOS and COX-2 expression are shown. Band intensity was quantified by densitometry and normalized to β-actin (** p < 0.01; *** p < 0.001) compared to the LPS-treated group.