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. 2020 May 25;13(5):104. doi: 10.3390/ph13050104

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of Amb4269951 on the expression of sphingomyelinases in U251MG cells. (A) Real-time PCR analysis of the mRNA expression of SMPD1-5, SMPDL3A, and SMPDL3B in U251MG cells. The percentage of relative mRNA expression is expressed as the ratio of the target mRNA to the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA. Each column represents the mean ± SD (n = 3). (B) Effect of Amb4269951 on the expression of SMPD4 protein in U251MG cells. Cells were incubated with various concentrations of Amb4269951 for 4 h. Western blot analysis shows the SMPD4 and ß-actin levels in whole cell lysates of drug-treated U251MG cells.