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Puffer fish livers tetrodotoxin (TTX) contents detected by LC-MS/MS. (a) MRM2 (m/z 320.00 to m/z 161.80) chromatograms of TTX standards and samples. (b) TTX content boxplot. Wilcoxon rank-sum test was applied for significance testing of average TTX content between toxic species (*** p-value < 0.001; NS: Non-Significant). STD: standard; TObs: T. obscurus; TXan: T. xanthopterus; TBim: T. bimaculatus; TOce: T. ocellatus; TOceH: hybrid suspected T. ocellatus.
