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. 2020 May 25;18(5):278. doi: 10.3390/md18050278

Figure 2.

Figure 2

Puffer fish gut microbial structure and diversities. (a) Operational taxonomic units (OTUs) relative abundance at Phylum level. Phyla with relative abundance less than 1% were assigned to “Others”. (b) Top ten OTUs relative abundance at latest taxonomic levels. Low relative abundance OTUs outside the top ten were assigned to “Others”. (c) Chao1 alpha diversity boxplot. Significant testing was conducted by ANOVA among species, and Welch’s T-test between toxic group (N = 24) and nontoxic group (N = 18). * p < 0.05. (d) Unweight UniFrac principle coordinate analysis. Hollow ellipses and solid ellipses represented clustering of samples grouped by puffer species and toxicity respectively (95% confidence). The significance was tested by PERMANOVA (999 times permutation). (e) ANCOM test volcano plot. Dots with Log2FC > 0/Log2FC < 0 represents OTUs in gut microbiota of toxic/non-toxic puffer species. Dashed line separated OTUs that were significant distinct (above) and non-significant (below). TObs: T. obscurus; TXan: T. xanthopterus; TBim: T. bimaculatus; TOce: T. ocellatus; TOceH: hybrid suspected T. ocellatus.