Figure 3.

Puffer fish gut microbial co-occurrence network analysis. (a) Co-occurrence network at OTU level. Co-occurrence pairs with Spearman rho threshold >0.8 and p-value < 0.05 were shown. Edges showed the positive (red) and negative (blue) relationships between nodes. Network centralities in each puffer group were highlighted. (b) Cladogram showing common tree of network centralities and their percentage relative abundance (%RLA) in gut of puffer species. The relative abundance of the centralities in toxic (N = 24) and non-toxic puffer (N = 18) species were compared by Wilcoxon rank sum test (WT) (* p < 0.05, ** p < 0.01, *** p < 0.001; NS: Non-significant). TObs: T. obscurus; TXan: T. xanthopterus; TBim: T. bimaculatus; TOce: T. ocellatus; TOceH: hybrid suspected T. ocellatus.