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. 2020 Apr 27;12(5):1088. doi: 10.3390/cancers12051088

Figure 3.

Figure 3

HMGCS1 enhances the tumor growth and lung metastasis of gastric cancer cells. (A,B) NCI-N87 cells were either transfected with the HMGCS1-expressing construct (HMGCS1) or empty vector (EV) (A) or infected with lentiviruses expressing siRNAs against HMGCS1 (#729) or luciferase (B). After treatment for 48 h, the treated cells were subcutaneously injected into nude mice (n = 4 per group) for measurement of tumor sizes at the time indicated (left). Bar, 0.5 cm. The mice were sacrificed on day 29 and subcutaneous tumors were excised for the detection of HMGCS1, Oct4, and SOX-2 transcripts using quantitative real-time PCR (right). Mean ± SD. (C) AGS cells were transfected with the HMGCS1-expressing construct or empty vector for 48 h and then injected into nonobese diabetic severe-combined immunodeficiency (NOD-SCID) mice (n = 4 per group) by tail vein injection for measurement of metastatic nodules in lungs. After 13 weeks, the mice were sacrificed and the metastatic nodules in lungs of the treated mice were also counted by gross and microscopic examination. Mean ± SD. *, p < 0.05; **, p < 0.01; ***, p < 0.001.