Figure 1.

Overview of RNA-seq-based gene fusion discovery workflow. The RNA was extracted from the tumor tissue and matched plasma of patients with glioblastoma (GBM) (n = 9) and plasma of healthy controls (n = 10) and was sequenced using the QIAseq Targeted RNAscan Human Oncology Panel. Schematic illustration of the workflow depicting the RNA extraction, library preparation, sequencing, data processing, analysis and final validation using droplet digital PCR and fusion annotation. GBM, glioblastoma; EV RNA, extracellular vesicle RNA; MT, molecular tag; SPE, single primer extension.