Figure 3.

Impact of gonotrophic cycle and time post infection on vertical transmission. ZIKV was quantified in single fourth instar larvae on the second or third gonotrophic cycle at 10 or 17 days post oral infection. (A) Oviposition rate per condition. The number of mosquito females that laid eggs was divided by the total number of females that were blood fed. N blood-fed mosquitoes >24. (B) Vertical transmission rate. The number of progeny with at least one infected larva was divided by the total number of tested progeny. N progeny >14. (A,B) Bars indicate arithmetic means ± s.e. (C) Filial infection rate. Each circle represents the infection rate calculated from 10 single larvae randomly selected from all larvae from one infected mother (one progeny). Bars indicate arithmetic means ± s.e.m. (D) ZIKV genomic RNA (gRNA) copies per infected larvae. Each point represents one larva. Bars indicate geometric means ± 95% CI. (C,D) Larvae for each condition were collected from at least 14 progeny. * p-value < 0.05; **, p-value < 0.01; ***, p-value < 0.001, as calculated by Z-test (A,B) and Tukey’s test (C,D). Number of samples analyzed in each condition are detailed in Supplementary Table S1.