Brucella abortus invades and replicates in T-HESC cells. (A) Non-decidualized (T-HESC) and decidualized (T-HESCd) endometrial cells were infected with wild type B. abortus and two isogenic mutants (virB10 and btpAbtpB), and colony forming unit (CFU) numbers of intracellular bacteria were determined at different times post-infection (p.i.). (B). Decidualized T-HESC were pretreated for 1 h with different doses of Colchicine (10, 5, 2.5 µM), Monodansylcadaverine (MDC; 200, 100, 5 μM), Cytochalasin D (2, 1, 0.5 µg/mL), or DMSO (vehicle) before infection with wild type B. abortus. Intracellular CFU were determined at 1 h p.i. Results are expressed as mean ± SD from three independent experiments run in duplicates. *** p < 0.001 versus control.