Figure 2.

B. abortus infection does not induce cytotoxicity or alterations in decidualization in T-HESC cells. Decidualized T-HESC cells were infected or not (NI) with B. abortus wild type or its isogenic btpAbtpB mutant, and culture supernatants were harvested at 24 and 48 h p.i. to measure the levels of prolactin by ELISA (a) and the activity of lactate dehydrogenase (LDH) using a commercial non-radioactive cytotoxicity assay (b). In the latter assay, a control of 100% cell lysis (Max) was obtained by hypotonic lysis of the same number of non-infected cells. Results are expressed as mean ± SD from three independent experiments run in duplicates. ns: non-significant versus NI.