Figure 3.

CYC31 activates the glycogen synthesis signaling in C2C12 myotubes: (A) Serum-starved C2C12 myotubes were treated with 2 µM CYC31 for 4 h and 8 h. For the insulin-treated group, cells were incubated with 10 nM insulin for 5 min. Then, p-GSK3β, p-GS, and p-Akt were detected by western blotting (B) The expression levels of p-Akt, p-GSK3β and p-GS: Band density was measured by Image J software and normalized to β-Actin. Data was shown as mean ± SD values (n = 3), ** p < 0.01, *** p < 0.001 compared with the DMSO-treated group.