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. 2020 May 13;12(5):1227. doi: 10.3390/cancers12051227

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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LINC00261 regulated E-cadherin expression. (a) The qPCR analysis of the genes commonly deregulated in both CRISPR systems, as shown in Figure 5e, in addition to FOXA2 (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, two-way ANOVA, unpaired t-test). (b) Expression of E-cadherin mRNA (upper panel) and protein levels (lower panel) in three wild-type and three promoter knockout clones (**** p < 0.0001, two-way ANOVA). (c) Brightfield images of wild-type and LINC00261 promoter knockout clones (20× objective, scale bar = 50 µm). (d) Cellular fractionation in lung cancer and pancreatic cancer cell lines, highlighting nuclear enrichment of LINC00261. (e) Relative luciferase activity of the CDH1 gene promoter constructs normalized to the pGL3-vector with a minimal cytomegalovirus (CMV) promotor (pGL3-minCMV). The average of the relative luciferase activity in wild-type clones was set to 1.0 (**** p < 0.0001, two-way ANOVA).