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. 2020 May 21;21:75–85. doi: 10.1016/j.omtn.2020.05.019

Figure 2.

Figure 2

RPL32 Silencing Inhibits the Growth of Lung Cancer Cells in a p53-Dependent Manner

(A) Cell numbers were counted after transfection with scramble siRNA or RPL32 siRNA for 96 h (n = 3; mean ± SD). **p < 0.01. (B) Immunoblot analysis of RPL32, p53, and p21 in A549 and NCI-H460 cells transfected with scramble siRNA or RPL32 siRNA. (C) Cell-cycle analysis of A549 and NCI-H460 cells transfected with scrambled siRNA or RPL32 siRNA. (D) Colony formation assay of A549 and NCI-H460 cells transfected with scramble siRNA or RPL32 siRNA. (E) Quantitative real-time PCR analysis of p53 mRNA levels in A549 and NCI-H460 cells transfected with RPL32 siRNA and p53 siRNA alone or together (n = 3; mean ± SD). **p < 0.01. (F) Cells were counted 96 h after transfection with RPL32 siRNA and p53 siRNA alone or together (n = 3; mean ± SD). **p < 0.01. (G) Immunoblot analysis of RPL32, p53, and p21 in cells transfected with RPL32 siRNA and p53 siRNA alone or together. (H) Representative histograms of cell-cycle analysis of cells transfected with RPL32 siRNA and p53 siRNA alone or together.