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IFA on mosquito midguts colonized with Asaia-GFP using anti-Asaia monoclonal antibody. (A) Bright-field and (B) corresponding fluorescent images showing the green signal from an Asaia strain expressing the green fluorescent protein (Asaia-GFP); (C) red-labelled bacteria recognized by anti-Asaia mAbs, and (D) the co-localization of the signals on the merged image. Microscopy magnification is 100X and scale bar = 5 μm for all panels.
