Figure 4.

Transcriptomic analysis investigating the response to the anti-Asaia antibody in male and female An. stephensi. Male and female An. stephensi mosquitoes were injected with the anti-Asaia or Herc antibody, and the transcriptomic response of each sex to Anti-Asaia vs. Herc antibody treatment was assessed. (A) Venn diagram indicating the number of genes up- or down-regulated in males only, females only, or both males and females in response to anti-Asaia treatment. (B,C) Molecular function Gene Ontology (GO) distribution of transcripts affected by anti-Asaia treatment in a male- or female-specific manner. GO assignment for differentially regulated transcripts and chart generation was performed in Blast2GO. GO assignments are “multi-level,” meaning all terminal GO terms (i.e., terms at terminal nodes in the directed acyclic graph) for each dataset are included, regardless of GO level. Numbers in parentheses indicate the number of sequences assigned to each GO term; note that individual sequences can belong to more than one GO category.