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. 2020 May 11;18(5):250. doi: 10.3390/md18050250

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of ultrasound assisted extraction (UAE) conditions (solvent concentration (30%, 50% and 70% ethanol), ultrasonic frequency (control, 35 kHz and 130 kHz) and UAE treatment time (10 and 30 min)) on the extract yield obtained from F. vesiculosus. Different letters indicate statistical differences (p < 0.05) on the yields of seaweed extract obtained using different solvents at each US frequency: control (m-n), 35 kHz (o-p) and 130 kHz (q-s). ^abc columns with similar letters are not significantly different (p < 0.05) treated for 10 min; ABC columns with similar letters are not significantly different (p < 0.05) treated for 30 min. The extraction yield is calculated by using following formula: Extraction yield (%) = (weight of dry extract / weight of dry sample) × 100.