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. 2020 May 14;12(5):1240. doi: 10.3390/cancers12051240

Figure 2.

Figure 2

Camptothecin sensitivity of colon cancer cell subpopulations with or without treatment with 4,5,6,7-tetrabromobenzotriazole (TBB). (A) Survival assay of the Fluorescence Activated Cell Sorting (FACS) sorted non-CSC-like (CD44 negative, “CD44 neg”, left graph) or CSC-like (CD44 positive, “CD44 pos”, right graph) cells of Caco2 after treatment with DMSO or 10 μM TBB. The sorted cells were plated and treated with DMSO or indicated concentrations of CPT for 72 h before the percentage of viable cells was measured using the PrestoBlue viability assay. Data were normalized to the viability of the cells without CPT (DMSO) and plotted as mean +/− standard error of the mean (SEM). **** p < 0.0001, *** p < 0.001; ** p < 0.01, Welch’s t-test, n = 6. (B) Same as (A) except that DLD1 cell subpopulations were analyzed. *** p < 0.001; ** p < 0.01; * p < 0.05, Welch’s t-test.