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. 2020 May 19;12(5):1284. doi: 10.3390/cancers12051284

Figure 2.

Figure 2

Diacylglycerol O-acyltransferase (DGAT)-dependent LD accumulation in dendritic cells (DCs) leads to metabolic reprogramming. DCs were incubated with non-conditioned medium (NCM) or treated for 2 or 3 days either with conditioned medium from AE17 or Ab1 mesothelioma cells maintained at pH 7.4 or pH 6.5 (7.4/CM and 6.5/CM, respectively). (A–C) Effects of 6.5/CM with or without delipidated serum (A), 15 µM A922500 (DGAT1i) (B), or 10 µM PF-06424439 (DGAT2i) (C) on cellular LD content, as determined using BODIPY 495/503 (n = 3, ** p < 0.01, *** p < 0.001; ns = non-significant). (D–G) Effects of 6.5/CM with or without 5 µM SB-431542 and either DGAT1i or DGAT2i on the extracellular acidification rate (ECAR) (D,E) and oxygen consumption rate (OCR) (F,G), as detected using the Seahorse XF Analyzer (n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns = non-significant).