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. 2020 May 19;12(5):1284. doi: 10.3390/cancers12051284

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The acidic mesothelioma milieu alters the ability of dendritic cell (DC)-based vaccine to induce anti-tumor T cell activation in vivo. (A–C) CD8⁺ splenocytes collected at day 14 post-vaccination (i.e., NCM, 6.5/CM, and 6.5/CM + 5 µM SB-431542, see Figure 5) were re-exposed to Ab1 mesothelioma cells. Proliferation of CD8⁺ splenocytes was detected based on CFSE dilution (n = 6, * p < 0.05, ** p < 0.01) (A) and activation was probed based on interferon gamma (IFN-γ) production (B) and lysosomal-associated membrane protein-1 (LAMP-1) staining (C) (n = 6, * p < 0.05, ** p < 0.01). (D) IFN-γ production in circulating CD4⁺ lymphocytes stimulated with phorbol myristate acetate (PMA)/ionomycin (n = 6, * p < 0.05, ** p < 0.01).