(a).
| Components | Volume (μL) |
|---|---|
| H2O | 17.1 |
| 10x buffer | 2.5 |
| MgCl2 (25 mM) | 1.5 |
| dNTPs (2.5 mM) | 0.5 |
| F3 (pmol) | 0.5 |
| B3 (pmol) | 0.5 |
| Taq polymerase (2 U) | 0.4 |
| Template DNA | 2 |
| Total | 25 |
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