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Reaction mixture used in Strong-LAMP assays. Bst polymerase, buffer, and MgSO4 were supplied by New England Biolabs, betaine by SIGMA, and the dNTPs by Intron.
| Component | Volume (μL) |
|---|---|
| H2O | 7.7 |
| Betaine (1 M) | 5 |
| MgSO4 | 1.5 |
| dNTPs (2.5 mM) | 3.5 |
| 10x buffer | 2.5 |
| FIP (40 pmol/μL) | 0.4 |
| BIP (40 pmol/μL) | 0.4 |
| F3 (5 pmol/μL) | 0.5 |
| B3 (5 pmol/μL) | 0.5 |
| Bst polymerase 2.0 | 1 |
| DNA | 2 |
| Total | 25 |
