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. 2020 Jun 8;19:1533033820928144. doi: 10.1177/1533033820928144

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — Sirtuin 1 was a Direct Target of miR-12129. A, MicroRNA-12129 binding site within the 3’-UTR of SIRT1 was predicted. B, Relative activities of luciferase reporters in NCI-N87 cell lines, C, SNU-5 cell lines. D, Pearson’s correlation analysis of the relative expression levels of miR-12129 and the relative SIRT1 mRNA levels in gastric cancer tissues (e) qRT-PCR analysis of SIRT1 expression in gastric cancer tissues (N = 30). F, Quantitative real-time polymerase chain reaction analysis of SIRT1 expression in gastric cancer cell lines. *P < .05 vs Control. miR indicates microRNA; qRT-PCR, quantitative real-time polymerase chain reaction; SIRT1, sirtuin 1; 3’-UTR, 3′ untranslated region.