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. 2020 Jun 8;19:1533033820928144. doi: 10.1177/1533033820928144

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — Knockdown of SIRT1 inhibited proliferation and cell cycle in Gastric cancer cells. A, Quantitative real-time polymerase chain reaction analysis of SIRT1 expression in gastric cancer cell lines. B, Western blot analysis of SIRT1 expression in gastric cancer cell lines. C, Proliferation ability was tested using the CCK-8 in NCI-N87 cell lines, (D) SNU-5 cell lines. The percentages of cells in G0/G1, S and G2/M phase in NCI-N87 cell lines, (E) SNU-5 cell lines. F, Images of cells in a cell cycle after transfection with miR-12129 mimics was assessed by Flow cytometry in NCI-N87 and SNU-5 cell lines. *P < .05 versus si-con. CCK-8 indicates Cell Counting Kit-8; con, control; miR, microRNA.