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. 2020 May 4;20(1):530–542. doi: 10.3892/etm.2020.8711

Figure 2.

Figure 2

Overexpression of gga-miR-375 inhibits proliferation and the cell cycle in DF-1 cells. DF-1 cells transfected with gga-miR-375 mimic decreases YAP1 (A) protein and (B) mRNA expression levels after 48 h of transfection. gga-miR-NC and mock are the control groups. (C) Overexpression of gga-miR-375 inhibits cell proliferation as indicated by Cell Counting Kit-8 assay results. (D) Overexpression of gga-miR-375 inhibits cell cycle progression as measured with a FACSCalibur flow cytometer. (E) Overexpression of gga-miR-375 downregulates the expression levels of YAP1, cyclin D1 and cyclin E. Data are presented as the mean ± SD of three independent experiments. **P<0.01. NC, negative control; miR, microRNA; YAP1, Yes-associated protein 1; OD, optical density; PI, propidium iodide.