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. 2020 May 4;20(1):530–542. doi: 10.3892/etm.2020.8711

Figure 3.

Figure 3

Knockdown of gga-miR-375 promotes proliferation and the cell cycle in DF-1 cells. (A) DF-1 cells transfected with anti-gga-miR-375 had decreased gga-miR-375 mRNA expression at 48 h following transcription. Anti-gga-miR-con group and mock group were used as the controls. DF-1 cells transfected with anti-gga-miR-375 exhibited increased (B) YAP1 protein and (C) mRNA expression levels compared with the control groups. (D) Overexpression of anti-gga-miR-375 promotes cell proliferation as indicated by Cell Counting Kit-8 assay results 24, 48 and 72 h after transfection. (E) Overexpression of anti-gga-miR-375 promotes the cell cycle as demonstrated by flow cytometry results. (F) DF-1 cells transfected with anti-gga-miR-375 had upregulated expression levels of YAP1, cyclin D1 and cyclin E. Anti-gga-miR-con and mock groups were used as the control groups. Data are presented as the mean ± SD of three independent experiments. **P<0.01 vs. anti-miR-con group. miR, microRNA; YAP1, Yes-associated protein 1; NC, negative control; con, control; OD, optical density.