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. 2020 May 4;20(1):530–542. doi: 10.3892/etm.2020.8711

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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OE of YAP1 promotes proliferation and cell cycle in DF-1 cells. (A) DF-1 cells transfected with pRK5-Flag-YAP1 were harvested after 48 h for western blot analysis with antibodies against YAP1 and β-actin. (B) OE of YAP1 promotes cell proliferation as identified by Cell Counting Kit-8 assay results 24, 48 and 72 h after transfection. (C) OE of YAP1 promotes the cell cycle, as demonstrated by flow cytometry. (D) OE of YAP1 promotes the protein expression levels of cyclin D1 and cyclin E after 48 h of transfection. Data are presented as the mean ± SD of three independent experiments. ^*^*P<0.01 vs. EV group. EV, empty vector; YAP1, Yes-associated protein 1; OD, optical density; OE, overexpression; PI, propidium iodide.