Metabolites concentration produced by probiotic strains, namely, (a) Lb. rhamnosus ŁOCK 1087, (c) Lb. paracasei ŁOCK 1091, (e) Lb. reuteri ŁOCK 1092, (g) Lb. plantarum ŁOCK 0860, and (i) Lb. pentosus ŁOCK 1094 in dependence on the used prebiotic (MD–maltodextrin, INU–inulin, CS–corn starch, AP–apple pectin, BG–β-glucan) or glucose (GLU) comparatively as a carbon source in the cultivation medium. Moreover, the correlations between used carbohydrates, as well as their concentrations, and the synthesized amount of metabolites, were presented as the PCA biplots, namely, plots (b,d,f,h,j) for above-mentioned strains, respectively.