Table 2.
Discrimination ability of standard and species-specific barcodes and their combinations
| DNA barcodes type | length (bp) | Polymorphic sites | Polymorphic sites (%) | Identified species (Success rate) | ||
|---|---|---|---|---|---|---|
| Standard DNA barcodes | nrITS | ITS1 + 5.8S | 449 | 6 | 1.34 | 0 (0%) |
| 5.8S + ITS2 | 467 | 16 | 3.43 | 1 (11%) | ||
| nrITS | 768 | 22 | 2.92 | 1 (11%) | ||
| plastid | matK | 1527 | 14 | 0.92 | 2 (22%) | |
| rbcL | 1428 | 8 | 0.56 | 2 (22%) | ||
| trnH-psbA | 386 | 23 | 5.96 | 2 (22%) | ||
| combination | matK + rbcL | 2955 | 22 | 0.74 | 3 (33%) | |
| trnH-psbA + matK + rbcL | 3341 | 44 | 1.32 | 3 (33%) | ||
| matK + rbcL + nrITS | 3723 | 44 | 1.18 | 5 (56%) | ||
| trnH-psbA + matK + rbcL + nrITS | 4109 | 66 | 1.61 | 4 (44%) | ||
| Genomic element | trnE-T | 995 | 21 | 2.11 | 1 (11%) | |
| trnT-L | 1026 | 36 | 3.51 | 2 (22%) | ||
| potential specific-barcodes | ndhF | 2244 | 38 | 1.69 | 5 (56%) | |
| ndhF-rpl32 | 993 | 28 | 2.82 | 4 (44%) | ||
| rpl32-trnL | 632 | 16 | 2.53 | 4 (44%) | ||
| trnC-petN | 900 | 82 | 9.11 | 4 (44%) | ||
| trnS-G | 1233 | 26 | 2.11 | 5 (56%) | ||
| Combination* | 5795 | 200 | 3.45 | 6 (67%) | ||
| Complete plastid genomes (only one IR) | 133,980 | 1151 | 0.86 | 7 (78%) | ||