Fig. 3.

Transcriptional regulation of ER-regulated genes by R269C-ER in breast and pancreatic cancer cells. MCF-7 (a, b) and Colo-357 cells (c, d) were transiently transfected with either WT-ER or R269C-ER constructs and treated with E2 (10nM) or a control vehicle for 24 h. mRNA levels of GREB-1 and TGF-α were determined 48 h after transfection by quantitative RT-PCR and are shown relative to the control WT-ER. The results are from a representative experiment of at least three independent experiments, each performed in triplicates, each bar represents the mean ± SD. *, P < 0.05, **, P < 0.01