Fig. 4.

AP-1 dependent transcriptional activity of R269C-ER. Cells were transiently transfected with either WT-ERα or R269C-ERα constructs together with the AP-1 luciferase reporter and treated with ICI 182780 (100 nM) or a control vehicle for 24 h. Luciferase activities were analyzed and normalized to Renilla luciferase units and are shown relative to the control WT-ER. The results are from a representative experiment of at least three independent experiments, each performed in hexaplicates, each bar represents the mean ± SD. *, P < 0.05, **, P < 0.01